pinonbistro's Identifications

Under an old Quercus robur on the side of the road. ITS sequence (based on the ITS4, Sanger) isn’t definitive and unfortunately the forward sequence (ITS1F) wasn’t successful. Culture and dried specimen available at the University of Stirling.

It appears to be closely related to C. albocyaneus from the output, but when looking deeper it could be caninus or C. pastoralis which Dima et al., 2021 were treating it as C. epsomiensis (as C. pastoralis, holotype).

VOUCHER # 73204

DATE: 28 NOV 2020

STATE: WA

COUNTY: PIERCE

FORAY ID: South Sound Fungal Diversity Project.

SITE NAME: Forested area just South of the Joint Base Lewis McChord Education Center.

iNat #: 66497034

NEARBY FLORA:

Conifer: Douglas Fir, Hemlock.
Deciduous: Vine Maple, Red Huckleberry.
Evergreen: Sword Fern, Oregon Grape, Rattlesnake Tongue Plantain.

SUBSTRATE: Wood Pieces (likely Conifer), Leaf Litter, Dense Humus Soil, Needle Duff, Moss, Grass.

HABIT: Few (5 within 3-foot area).

LIGHT EXPOSURE: Shade - Partial Shade.

MOISTURE: Wet, .5 inches of cumulative rain in previous 4 days

AMBIENT TEMP: 46 Degrees F

SOIL TEMP: 40 Degrees F

ECOSYSTEM: 2nd-&-3rd Growth Conifer Forest

ELEVATION: 315 Ft/96 Meters

LAY OF THE LAND: Flat, Slightly Irregular

ODOR: Pleasant Mushroom.

TASTE: Unknown, ostensibly Pleasant Mushroom.

REFERENCES:

Mushrooms of the Redwood Coast (Siegel & Schwarz), pg 558;
Mushrooms of the Pacific Northwest (Trudell & Ammirati), pg 280;
Pictorial Key to Pacific NW Mushrooms (Danny Miller)
Mushroom Expert Website (Michael Kuo)

NOTE: There is much discussion in all of these references about the “similarities” of this species w/G. ambigua. The preponderance of the differences are apparently microscopic, but…..there is a distinct difference in Ecosystems…Pine & Sand vs Conifer & Moss substrates.

Hence, in my opinion it would be very-much worthwhile to have these specimens, individually, DNA analyzed. Who knows….there might be 2 different species in this sample.

VOUCHER SPECIMENs SENT TO:

ALDEN DIRKS
U of Michigan
Dept of Ecology & Evolutionary Biology
Ann Arbor, MI

DNA: Confirmed Gyromitra infula.
AAGGATCATTACCATAATGTGCGCTGTCACACGAGTCATCACGCCTGGGC
AGTAATTCCCGCCATATAGTAGGGCAAAAGGCCACCCGGAGTGCCCCCGA
AACAAAACTGTCGGGGGGAGGGCTGCAGTGTCAGCTGGGCTCCGGGGGCG
GGATAGAGTTAGAGTCCCGTCCGCGTCACGCAAGAATCGAAATCGAACTT
GAACCCGGTGCTGGGTTTGCCCGTTAAGGCTGATTGGCCCGTTCACCTTC
ACAAAACCCTCCGTGTACCATTTCCCTGTTGCTTCCCCGCGCCCCCGCGG
CGCGGGGGAGGTCCACCTGAAACACAACAGCACCAAACTGCAGTCAGAAA
AACAAACAAACAATAAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCC
GCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAA
TTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCC
GGAGGGCATGCCTGTTCGAGCCTCAATGAAACCGCTCCCCGTGCGGCCGT
TCCTCGAACGTCCCCGGGGGTAGTGGCGGACGCGAACGCCCCCAAAAGCG
TGCGCGCCGCTCAAATCCGCGGTCCGCAGGCCCGAGGGCGCACCCGACGT
AGTGATGAATTTTCTCGTCGACGTGCGACCTCAAGCGCCTGGGACCTTAA
GCGCTCCGGCGCCGCTTACAATCGGTTGAGCTCGGATCAGGTAGG